GENETIC-ANALYSIS OF IONIZING-RADIATION SENSITIVE MUTANTS OF CULTURED MAMMALIAN-CELL LINES

被引：89

作者：

JEGGO, PA ^{[1
]}

TESMER, J ^{[1
]}

CHEN, DJ ^{[1
]}

机构：

[1] UNIV CALIF LOS ALAMOS SCI LAB,GENET GRP,LOS ALAMOS,NM 87544

来源：

MUTATION RESEARCH | 1991年 / 254卷 / 02期

关键词：

IONIZING RADIATION SENSITIVE MUTANTS; MAMMALIAN CELL LINES; CULTURED; HYBRIDS; CONSTRUCTION; HAT; OUABAIN;

D O I：

10.1016/0921-8777(91)90003-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genetic diversity of a range of ionising radiation sensitive mutants of cultured mammalian cell lines has been examined. Hybrids were constructed from suitably marked diploid cells by cell fusion and selected using resistance to HAT and ouabain. Hybrids were examined for ploidy and gamma-ray sensitivity. The data suggest that at least 8 and possibly 9 complementation groups exist which confer sensitivity to ionising radiation. Mutants in at least 3 distinct complementation groups have a reduced ability to rejoin DNA double-strand breaks.

引用

页码：125 / 133

页数：9

共 33 条

[1] LARGE-SCALE ISOLATION OF UV-SENSITIVE CLONES OF CHO CELLS
BUSCH, DB
CLEAVER, JE
GLASER, DA
[J]. SOMATIC CELL GENETICS, 1980, 6 (03): : 407 - 418
[2] POLYETHYLENE GLYCOL-INDUCED MAMMALIAN-CELL HYBRIDIZATION - EFFECT OF POLYETHYLENE-GLYCOL MOLECULAR-WEIGHT AND CONCENTRATION
DAVIDSON, RL
OMALLEY, KA
WHEELER, TB
[J]. SOMATIC CELL GENETICS, 1976, 2 (03): : 271 - 280
[3] GENETIC HETEROGENEITY OF XERODERMA PIGMENTOSUM DEMONSTRATED BY SOMATIC CELL HYBRIDIZATION
DEWEERDK.EA
BOOTSMA, D
KEIJZER, W
[J]. NATURE-NEW BIOLOGY, 1972, 238 (81): : 80 - &
[4] A CHINESE-HAMSTER OVARY CELL-LINE HYPERSENSITIVE TO IONIZING-RADIATION AND DEFICIENT IN REPAIR REPLICATION
FULLER, LF
PAINTER, RB
[J]. MUTATION RESEARCH, 1988, 193 (02): : 109 - 121
[5] CELL CYCLE-DEPENDENT REPAIR OF DOUBLE-STRAND DNA BREAKS IN A GAMMA-RAY-SENSITIVE CHINESE-HAMSTER CELL
GIACCIA, A
WEINSTEIN, R
HU, J
STAMATO, TD
[J]. SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (05) : 485 - 491
[6] DNA-REPAIR ENZYME DEFICIENCY AND INVITRO COMPLEMENTATION OF THE ENZYME-ACTIVITY IN CELL-FREE-EXTRACTS FROM ATAXIA TELANGIECTASIA FIBROBLASTS
INOUE, T
YOKOIYAMA, A
KADA, T
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 655 (01) : 49 - 53
[7] GENETIC-HETEROGENEITY IN ATAXIA-TELANGIECTASIA STUDIED BY CELL-FUSION
JASPERS, NGJ
BOOTSMA, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (08): : 2641 - 2644
[8] GENETIC-ANALYSIS OF X-RAY-SENSITIVE MUTANTS OF THE CHO CELL-LINE
JEGGO, PA
[J]. MUTATION RESEARCH, 1985, 146 (03): : 265 - 270
[9] X-RAY-SENSITIVE MUTANTS OF CHINESE-HAMSTER OVARY CELL-LINE - ISOLATION AND CROSS-SENSITIVITY TO OTHER DNA-DAMAGING AGENTS
JEGGO, PA
KEMP, LM
[J]. MUTATION RESEARCH, 1983, 112 (06): : 313 - 327
[10] 6 COMPLEMENTATION GROUPS FOR IONIZING-RADIATION SENSITIVITY IN CHINESE-HAMSTER CELLS
JONES, NJ
COX, R
THACKER, J
[J]. MUTATION RESEARCH, 1988, 193 (02): : 139 - 144

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