INDUCTION OF THROMBOXANE SYNTHASE AND PROSTAGLANDIN ENDOPEROXIDE SYNTHASE MESSENGER-RNAS IN HUMAN ERYTHROLEUKEMIA-CELLS BY PHORBOL ESTER

被引：33

作者：

IHARA, H

YOKOYAMA, C

MIYATA, A

KOSAKA, T

NUSING, R

ULLRICH, V

TANABE, T

机构：

[1] NATL CARDIOVASC CTR,RES INST,DEPT PHARMACOL,5-7-1 FUJISHIRO DAI,SUITA,OSAKA 565,JAPAN

[2] UNIV CONSTANCE,FAC BIOL,W-7750 CONSTANCE,GERMANY

来源：

FEBS LETTERS | 1992年 / 306卷 / 2-3期

关键词：

THROMBOXANE SYNTHASE; PROSTAGLANDIN ENDOPEROXIDE SYNTHASE; 12-O-TETRADECANOYL-PHORBOL-13-ACETATE;

D O I：

10.1016/0014-5793(92)80990-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of 12-O-tetradecanoyl-phorbol- 13-acetate (TPA) on the mRNA levels of two enzymes, thromboxane synthase (TXS) and prostaglandin endoperoxide synthase (PES), responsible for the synthesis of thromboxane A, from arachidonic acid, were studied in human erythroleukemia (HEL) cells by RNA blot analysis. TPA induced both TXS and PES mRNAs in HEL cells in a dose-dependent manner at 36 h. The half-maximal and maximal effects for the induction of both mRNAs were at approximately 3x10(-9) M and at 10(-8) M, respectively. TXS and PES mRNA levels increased in a time-dependent fashion by TPA, and reached to 7- and 3.5-fold of the control, respectively after 48 h of TPA treatment. These results suggest that expression of TXS and PES genes in HEL cells were simultaneously stimulated by TPA.

引用

页码：161 / 164

页数：4

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