EVALUATION OF DNA-FINGERPRINTING BY PFGE AS AN EPIDEMIOLOGIC TOOL FOR SALMONELLA INFECTIONS

被引：58

作者：

MURASE, T ^{[1
]}

OKITSU, T ^{[1
]}

SUZUKI, R ^{[1
]}

MOROZUMI, H ^{[1
]}

MATSUSHIMA, A ^{[1
]}

NAKAMURA, A ^{[1
]}

YAMAI, S ^{[1
]}

机构：

[1] NATL INST HLTH,DEPT BACTERIOL,SHINJYUKU KU,TOKYO 162,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1995年 / 39卷 / 09期

关键词：

PULSED-FIELD GEL ELECTROPHORESIS (PFGE); MOLECULAR EPIDEMIOLOGY; EPIDEMIOLOGIC TOOL; SALMONELLA;

D O I：

10.1111/j.1348-0421.1995.tb03255.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bin I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another, There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study, These results suggest that the Bin I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.

引用

页码：673 / 676

页数：4

共 13 条

[1] EVALUATION OF THE MOLECULAR EPIDEMIOLOGY OF AN OUTBREAK OF MULTIPLY RESISTANT SHIGELLA-SONNEI IN A DAY-CARE-CENTER BY USING PULSED-FIELD GEL-ELECTROPHORESIS AND PLASMID DNA ANALYSIS [J].

BRIAN, MJ ;

VAN, R ;

TOWNSEND, I ;

MURRAY, BE ;

CLEARY, TG ;

PICKERING, LK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (08) :2152-2156

[2] THE PLASMID PATTERN AS AN EPIDEMIOLOGIC TOOL FOR SALMONELLA-TYPHIMURIUM EPIDEMICS - COMPARISON WITH THE LYSOTYPE [J].

BRUNNER, F ;

MARGADANT, A ;

PEDUZZI, R ;

PIFFARETTI, JC .

JOURNAL OF INFECTIOUS DISEASES, 1983, 148 (01) :7-11

[3] MOLECULAR CHARACTERIZATION OF VIBRIO-CHOLERAE O1 STRAINS BY PULSED-FIELD GEL-ELECTROPHORESIS [J].

CAMERON, DN ;

KHAMBATY, FM ;

WACHSMUTH, IK ;

TAUXE, RV ;

BARRETT, TJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (07) :1685-1690

[4] NEW BIOTYPING SCHEME FOR SALMONELLA-TYPHIMURIUM AND ITS PHYLOGENETIC SIGNIFICANCE [J].

DUGUID, JP ;

ANDERSON, ES ;

ALFREDSSON, GA ;

BARKER, R ;

OLD, DC .

JOURNAL OF MEDICAL MICROBIOLOGY, 1975, 8 (01) :149-166

[5]

KAUFFMAN F, 1954, ENTEROBACTERIACEAE

[6] THE XBAI-BLNI-CEUI GENOMIC CLEAVAGE MAP OF SALMONELLA-TYPHIMURIUM-LT2 DETERMINED BY DOUBLE DIGESTION, END LABELING, AND PULSED-FIELD GEL-ELECTROPHORESIS [J].

LIU, SL ;

HESSEL, A ;

SANDERSON, KE .

JOURNAL OF BACTERIOLOGY, 1993, 175 (13) :4104-4120

[7] A PHYSICAL MAP OF THE SALMONELLA-TYPHIMURIUM LT2 GENOME MADE BY USING XBAI ANALYSIS [J].

LIU, SL ;

SANDERSON, KE .

JOURNAL OF BACTERIOLOGY, 1992, 174 (05) :1662-1672

[8]

MURASE M, 1988, J JPN ASS INFECT DIS, V62, P164

[9] COMPARISON OF GENOMIC DNAS OF DIFFERENT ENTEROCOCCAL ISOLATES USING RESTRICTION ENDONUCLEASES WITH INFREQUENT RECOGNITION SITES [J].

MURRAY, BE ;

SINGH, KV ;

HEATH, JD ;

SHARMA, BR ;

WEINSTOCK, GM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) :2059-2063

[10]

NADA T, 1992, J JPN ASS INFECT DIS, V66, P465

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