INFECTIVITY OF PINE AND EUCALYPT ISOLATES OF PISOLITHUS-TINCTORIUS (PERS) COKER AND COUCH ON ROOTS OF EUCALYPTUS-UROPHYLLA BLAKE,S.T. INVITRO

被引:46
作者
LEI, J
LAPEYRIE, F
MALAJCZUK, N
DEXHEIMER, J
机构
[1] INRA, CTR RECH FORESTIERES NANCY, F-54280 SEICHAMPS, FRANCE
[2] CSIRO, DIV FORESTRY & FOREST PROD, WEMBLY, WA 6014, AUSTRALIA
[3] UNIV NANCY 1, BIOL LIGNEUX LAB, F-54506 VANDOEUVRE LES NANCY, FRANCE
关键词
acid phosphatase; Ectomycorrhiza; Eucalyptus urophylla; glycoprotein fibrils; Pisolithus tinctorius;
D O I
10.1111/j.1469-8137.1990.tb00516.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An ultrastructural comparison of early stages of mycorrhiza formation on Eucalyptus urophylla was carried out using two isolates of P. tinctorius, one cultured from a sporocarp collected under pine and the other cultured from a sporocarp collected under eucalypt. Major differences at the fungus–root interface were observed 2 and 4 d following inoculation. Eucalypt root cells in contact with the isolate from pine developed a thickened cell wall, whereas the isolate from eucalypt was associated with an accumulation of extracellular fibrils. The fibrils stained positively for carbohydrates and protein. They were absent with the isolate from pine. Four days after inoculation with the isolate from eucalypt, acid phosphatase activity was detected along the fungal plasmalemma at the interface with host cells. This enzyme activity was not detected with the isolate from pine. Vacuolar acid phosphatase activity was detected in both fungal isolates when in contact with Eucalyptus roots. It is suggested that host recognition of ectomycorrhizal symbionts can be manifested within hours of inoculation with the fungal partner. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:115 / 122
页数:8
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