CLONING AND NUCLEOTIDE-SEQUENCES OF THE COMPLEMENTARY AND GENOMIC DNAS FOR THE ALKALINE PROTEASE FROM ACREMONIUM-CHRYSOGENUM

被引：25

作者：

ISOGAI, T ^{[1
]}

FUKAGAWA, M ^{[1
]}

KOJO, H ^{[1
]}

KOHSAKA, M ^{[1
]}

AOKI, H ^{[1
]}

IMANAKA, H ^{[1
]}

机构：

[1] FUJISAWA PHARMACEUT CO LTD,RES & DEV GRP,YODOGAWA KU,OSAKA 532,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 02期

关键词：

D O I：

10.1080/00021369.1991.10870614

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Complementary DNA encoding Ac. chrysogenum alkaline protease (Alp) was isolated from the Ac. chrysogenum ATCC11550 cDNA library by express-blot assay. The genomic DNAs encoding Ac. chrysogenum Alp were isolated from the Ac. chrysogenum genomic DNA library using the cloned cDNA as a probe. The 3150 nucleotides of the gene were sequenced. The prepro-Alp consists of 402 amino acids and two intervening sequences are found within the coding region. The amino acid sequence of Ac. chrysogenum Alp has 57% homology to that of Aspergillus oryzae Alp. The entire cDNA, encoding Ac. chrysogenum Alp, when introducing into the yeast Saccharomyces cerevisiae, directed the secretion of enzymatically active Alp into the culture medium.

引用

页码：471 / 477

页数：7

共 28 条

[1] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[2]

Clemens M, 1984, TRANSCRIPTION TRANSL, P231

[3]

Davis RW, 1980, ADV BACTERIAL GENETI

[4] PLASMID-ENCODED HYGROMYCIN-B RESISTANCE - THE SEQUENCE OF HYGROMYCIN-B PHOSPHOTRANSFERASE GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND SACCHAROMYCES-CEREVISIAE [J].

GRITZ, L ;

DAVIES, J .

GENE, 1983, 25 (2-3) :179-188

[5] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[6]

Huynh TV, 1985, DNA CLONING TECHNIQU, V1, P49

[7]

IKENAKA T, 1976, TANPAKUSHITSU NO KAG, V2, P177

[8]

ISOGAI T, 1987, AGR BIOL CHEM TOKYO, V51, P2321

[9] POINT MUTATIONS DEFINE A SEQUENCE FLANKING THE AUG INITIATOR CODON THAT MODULATES TRANSLATION BY EUKARYOTIC RIBOSOMES [J].

KOZAK, M .

CELL, 1986, 44 (02) :283-292

[10] ARE SNRNPS INVOLVED IN SPLICING [J].

LERNER, MR ;

BOYLE, JA ;

MOUNT, SM ;

WOLIN, SL ;

STEITZ, JA .

NATURE, 1980, 283 (5743) :220-224

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