ASSAY OF THE C-ERBB-2 ONCOGENE ENCODED PROTEIN BY ELISA AND IMMUNOCYTOCHEMISTRY IN HUMAN BREAST-CANCER

被引：5

作者：

NUGENT, A

GALLAGHER, J

DOLAN, J

OHIGGINS, N

DUFFY, MJ

机构：

[1] ST VINCENTS HOSP,DEPT NUCL MED,DUBLIN 4,IRELAND

[2] ST VINCENTS HOSP,DEPT PATHOL,DUBLIN 4,IRELAND

[3] ST VINCENTS HOSP,DEPT SURG,DUBLIN 4,IRELAND

[4] UNIV DUBLIN TRINITY COLL,DEPT ANAT,DUBLIN 2,IRELAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1994年 / 31卷

关键词：

METHOD COMPARISON; CUTOFF POINTS;

D O I：

10.1177/000456329403100210

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The c-erbB-2 gene is amplified in a wide variety of different adenocarcinomas. Generally, gene amplification correlates with increased expression of the c-erbB-2 oncoprotein. Previous assays for the c-erbB-2 oncoprotein have been qualitative or semi-quantitative. In this investigation using human breast cancers, c-erbB-2 oncoprotein levels as measured by enzyme-linked immunosorbent assay (ELISA) correlated significantly with semi-quantitation by immunocytochemistry (r = 0.843, P < 0.0001, n = 97). The cut-off point for the ELISA which gave the strongest agreement with immunocytochemistry was 15 units/mu g protein. It is concluded that detection of c-erbB-2 oncoprotein by ELISA is quantitative and objective.

引用

页码：171 / 173

页数：3

共 14 条

[1]

CLARK GM, 1991, CANCER RES, V51, P944

[2]

GARCIA I, 1989, CANCER RES, V49, P6675

[3] USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES [J].