POLYADENYLYLATION DESTABILIZES THE RPSO MESSENGER-RNA OF ESCHERICHIA-COLI

被引：128

作者：

HAJNSDORF, E ^{[1
]}

BRAUN, F ^{[1
]}

HAUGELNIELSEN, J ^{[1
]}

REGNIER, P ^{[1
]}

机构：

[1] INST BIOL PHYSICOCHIM,F-75005 PARIS,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 09期

关键词：

RNA DECAY; POLY(A) POLYMERASE; POLY(A) TAILS; 3' EXORIBONUCLEASES; RIBOSOMAL PROTEIN S15;

D O I：

10.1073/pnas.92.9.3973

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The rpsO mRNA, encoding ribosomal protein S15, is only partly stabilized when the three ribonucleases implicated in its degradation-RNase E, polynucleotide phosphorylase, and RNase II-are inactivated, In the strain deficient for RNase E and 3'-to-5' exoribonucleases, degradation of this mRNA is correlated with the appearance of posttranscriptionally elongated molecules. We report that these elongated mRNAs harbor poly(A) tails, most of which are fused downstream of the 3'-terminal hairpin at the site where transcription terminates, Poly(A) tails are shorter in strains containing 3'-to-5' exoribonucleases. Inactivation of poly(A) polymerase I (pcnB) prevents polyadenylylation and stabilizes the rpsO mRNA if RNase E is inactive, In contrast polyadenylylation does not significantly modify the stability of rpsO mRNA undergoing RNase E-mediated degradation.

引用

页码：3973 / 3977

页数：5

共 23 条

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