OXYGEN MEDIATED PHOTOSYSTEM-I ACTIVITY IN THYLAKOID MEMBRANES MONITORED BY A PHOTOELECTROCHEMICAL CELL

被引：22

作者：

GOETZE, DC ^{[1
]}

CARPENTIER, R ^{[1
]}

机构：

[1] UNIV QUEBEC, CTR RECH PHOTOBIOPHYS, CP 500, TROIS RIVIERES GA9 5H7, QUEBEC, CANADA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1990年 / 52卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1990.tb01826.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— A photoelectrochemical cell has been used to monitor the effects of three enzymes on the photocurrent produced by isolated spinach thylakoids. The enzymes were glucose oxidase, superoxide dismutase and catalase. It is shown that all three inhibit the photocurrent to varying degrees. The results demonstrate that electron transport to the working electrode is mediated by oxygen. Further, the activity monitored originated from photosystem I with oxygen as the acceptor and photosystem II/plastoquinone as the donor. Thus, the photoelectrochemical cell constitutes a potential new approach for the monitoring of pseudocyclic electron transport. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1057 / 1060

页数：4

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