NEUTROPHIL-MEDIATED INFLAMMATORY RESPONSE IN MURINE LUPUS

被引:25
作者
JOSEFSSON, E
CARLSTEN, H
TARKOWSKI, A
机构
[1] Department of Clinical Immunology and Rheumatology, University of Goteborg
基金
英国医学研究理事会;
关键词
INFLAMMATION; POLYMORPHONUCLEAR LEUKOCYTES; T-LYMPHOCYTES; SYSTEMIC LUPUS ERYTHEMATOSUS; MRL 1PR/1PR MICE; DELAYED-TYPE HYPERSENSITIVITY;
D O I
10.3109/08916939309077373
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have studied the ability of the lupus prone MRL lpr/lpr (MRL/lpr) and (NZBxNZW)F, (NZB/W) female mice to raise granulocyte mediated inflammatory responses. These autoimmune strains, known to exhibit severe anergy as concerns T cell dependent immune function, are not well analysed with respect to neutrophil-mediated inflammatory responses. An in vivo model of granulocyte mediated inflammation has been developed in our laboratory. A single intradermal injection of olive oil into mouse footpad induces massive infiltration of polymorphonuclear cells (PMNC) within 24 h. This extravasation of PMNC gives rise to a localized footpad swelling, which can be easily and reproducibly measured and relates to severity of the inflammatory process. T cell independence of this inflammatory model was ascertained by in vivo T cell depletion using monoclonal antibodies to CD4 and CD8 molecules. Olive oil triggered inflammation was inducible in both young and aged lupus mice. The intensity of foot-pad swelling upon olive oil injection was similar in lupus mice and in healthy control strains. In contrast, aged MRL/lpr and NZB/W mice showed severely depressed T cell dependent inflammatory responses as assessed by delayed type hypersensitivity reaction to sheep red blood cells. We conclude that the PMNC mediated inflammatory potential is not affected in severely diseased lupus mice. The increased numbers of circulating PMNC together with intact PMNC function may explain why severely immune deficient lupus mice seldom show clinical signs of bacterial infection.
引用
收藏
页码:251 / 257
页数:7
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