A CAPILLARY ELECTROPHORESIS ASSAY FOR THE FIBRINOLYTIC AGENT, EMINASE

被引：6

作者：

BIRRELL, HC ^{[1
]}

BRIGHTWELL, MD ^{[1
]}

CAMILLERI, P ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,WELWYN GARDEN CIT AL6 9AR,HERTS,ENGLAND

来源：

CHROMATOGRAPHIA | 1994年 / 39卷 / 5-6期

关键词：

CAPILLARY ELECTROPHORESIS; PROTEINS; QUANTITATIVE ANALYSIS; EMINASE;

D O I：

10.1007/BF02274521

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary eIectrophoresis (CE) has been used to develop a method that allows analysis for all the main components of Eminase. This technique has also resolved the two glycosylated forms of lys-plasminogen. Quantitative CE analysis of concentration levels of this enzyme complex have been directly compared to those obtained from a bio-assay. A linear relation between the two methods has been demonstrated. Moreover, the CE method is highly reproducible, combines precision with speed, and allows quantitative analysis of the other protein components (streptokinase and human serum albumin) in Eminase.

引用

页码：325 / 328

页数：4

共 5 条

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[3]

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[5]

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