Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter

被引：42

作者：

OBrien, RM ^{[1
]}

Printz, RL ^{[1
]}

Halmi, N ^{[1
]}

Tiesinga, JJ ^{[1
]}

Granner, DK ^{[1
]}

机构：

[1] VANDERBILT UNIV,SCH MED,DEPT PHYSIOL & MOLEC BIOPHYS,NASHVILLE,TN 37232

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1264卷 / 03期

关键词：

PEPCK; insulin; gene transcription; (human);

D O I：

10.1016/0167-4781(95)00194-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoenolpyruvate carboxykinase (PEPCK) catalyses the rate limiting step in hepatic and renal gluconeogenesis. Glucagon (acting via cyclic AMP (cAMP)) and glucocorticoids stimulate PEPCK gene transcription, whereas insulin has the opposite effect. Since these are the major regulatory hormones controlling glucose homeostasis, and because increased hepatic glucose production is one of the characteristics of non-insulin dependent diabetes mellitus (NIDDM), investigators have speculated that the regulation of PEPCK gene expression may be defective in patients with NIDDM. To begin to investigate this possibility we have isolated and sequenced the human PEPCK gene promoter. In addition, we have constructed and analyzed a human PEPCK promoter-chloramphenicol acetyltransferase (CAT) fusion gene in an effort to correlate differences between the rat and human promoter sequences and the hormonal regulation of transcription.

引用

页码：284 / 288

页数：5

共 24 条

[1]

BEALE EG, 1985, J BIOL CHEM, V260, P748

[2] THE TRIUMVIRATE - BETA-CELL, MUSCLE, LIVER - A COLLUSION RESPONSIBLE FOR NIDDM [J].

DEFRONZO, RA .

DIABETES, 1988, 37 (06) :667-687

[3] REGULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE-EXPRESSION BY INSULIN - USE OF THE STABLE TRANSFECTION APPROACH TO LOCATE AN INSULIN RESPONSIVE SEQUENCE [J].

FOREST, CD ;

OBRIEN, RM ;

LUCAS, PC ;

MAGNUSON, MA ;

GRANNER, DK .

MOLECULAR ENDOCRINOLOGY, 1990, 4 (09) :1302-1310

[4] C-ERBA PROTOONCOGENES MEDIATE THYROID HORMONE-DEPENDENT AND INDEPENDENT REGULATION OF THE RAT GROWTH-HORMONE AND PROLACTIN GENES [J].

FORMAN, BM ;

YANG, CR ;

STANLEY, F ;

CASANOVA, J ;

SAMUELS, HH .

MOLECULAR ENDOCRINOLOGY, 1988, 2 (10) :902-911

[5] IDENTIFICATION OF A RECEPTOR FOR THE MORPHOGEN RETINOIC ACID [J].

GIGUERE, V ;

ONG, ES ;

SEGUI, P ;

EVANS, RM .

NATURE, 1987, 330 (6149) :624-629

[6] MOLECULAR PHYSIOLOGY AND GENETICS OF NIDDM - IMPORTANCE OF METABOLIC STAGING [J].

GRANNER, DK ;

OBRIEN, RM .

DIABETES CARE, 1992, 15 (03) :369-395

[7]

Hanson R W, 1994, Adv Enzymol Relat Areas Mol Biol, V69, P203

[8] CHARACTERIZATION OF A COMPLEX GLUCOCORTICOID RESPONSE UNIT IN THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE [J].

IMAI, E ;

STROMSTEDT, PE ;

QUINN, PG ;

CARLSTEDTDUKE, J ;

GUSTAFSSON, JA ;

GRANNER, DK .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (09) :4712-4719

[9] A RETINOIC ACID RESPONSE ELEMENT IS PART OF A PLEIOTROPIC DOMAIN IN THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE [J].

LUCAS, PC ;

OBRIEN, RM ;

MITCHELL, JA ;

DAVIS, CM ;

IMAI, E ;

FORMAN, BM ;

SAMUELS, HH ;

GRANNER, DK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2184-2188

[10]

MAURER RA, 1989, J BIOL CHEM, V264, P6870

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