PRODUCTION OF STABLE HUMAN-MOUSE HYBRIDOMAS SECRETING MONOCLONAL-ANTIBODIES AGAINST RH-D-ANTIGENS AND C-ANTIGENS

被引：11

作者：

RAPAILLE, A

FRANCOISGERARD, C

DONNAY, D

SONDAGTHULL, D

机构：

[1] CHU LIEGE,CTR TRANSFUS SANGUINE,CROIX ROUGE BELGIQUE,B 35,NIV 0,B-4000 LIEGE,BELGIUM

[2] STATE UNIV LIEGE,B-4000 LIEGE,BELGIUM

来源：

VOX SANGUINIS | 1993年 / 64卷 / 03期

关键词：

D O I：

10.1111/j.1423-0410.1993.tb05155.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Peripheral blood lymphocytes from donors immunized against Rh antigens were fused with mouse myelomas and heteromyelomas in order to obtain human-mouse hybridomas secreting antibodies specific for these antigens. Three cell lines secreting anti-D IgG and two secreting anti-c IgM were stabilized and produced immunoglobulins for several months. These human monoclonal antibodies were evaluated as reagents for Rh phenotyping. Their complementary activity towards weak D and partial D antigens is examined.

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页码：161 / 166

页数：6

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