A PUTATIVE ANAEROBIC COPROPORPHYRINOGEN-III OXIDASE IN RHODOBACTER-SPHAEROIDES .2. ANALYSIS OF A REGION OF THE GENOME ENCODING HEMF AND THE PUC OPERON

被引：40

作者：

GIBSON, LCD ^{[1
]}

MCGLYNN, P ^{[1
]}

CHAUDHRI, M ^{[1
]}

HUNTER, CN ^{[1
]}

机构：

[1] UNIV SHEFFIELD,KREBS INST,DEPT MOLEC BIOL,WESTERN BANK,POB 594,FIRTH COURT,SHEFFIELD S10 2UH,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 21期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01773.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The puc operon of Rhodobacter sphaeroides encoding polypeptides of the major light-harvesting complex, LH2, has been found to be linked to hemF, a gene encoding a putative anaerobic coproporphyrinogen III oxidase. The puc-hemF region of the R. sphaeroides genome has been investigated by insertional mutagenesis, complementation analysis of these insertional mutants and DNA sequencing. A third gene, designated pucC, has been found immediately downstream of pucA and has been shown to be essential for LH2 expression. pucC is cotranscribed with pucB and pucA; however, hemF and the pucBAC operon were found not to be transcriptionally linked. Ultrastructural studies indicated that the morphology of the intracytoplasmic membrane may depend upon expression of pucC as well as pucBA.

引用

页码：3171 / 3186

页数：16

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