THE N-LINKED CARBOHYDRATE CHAIN OF THE 85-KILODALTON GLYCOPROTEIN FROM TRYPANOSOMA-CRUZI TRYPOMASTIGOTES CONTAINS SIALYL, FUCOSYL AND GALACTOSYL (ALPHA-1-3)GALACTOSE UNITS

被引:55
作者
COUTO, AS
GONCALVES, MF
COLLI, W
DELEDERKREMER, RM
机构
[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT QUIM ORGAN,PABELLON 2,CIUDAD UNIVERSITARIA,RA-1428 BUENOS AIRES,ARGENTINA
[2] UNIV SAO PAULO,INST QUIM,DEPT BIOQUIM,SAO PAULO,SP,BRAZIL
基金
巴西圣保罗研究基金会;
关键词
Gal(α1-3)gal; Glycoprotein; Tc-85; Trypanosoma cruzi;
D O I
10.1016/0166-6851(90)90012-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [14C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [14C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3H-labeled by the galactose oxidase-NaB3H4 method. [3H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [3H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990.
引用
收藏
页码:101 / 108
页数:8
相关论文
共 19 条
  • [1] ALVES MJM, 1986, MOL BIOCHEM PARASIT, V21, P75, DOI 10.1016/0166-6851(86)90081-2
  • [2] MAPPING OF SURFACE GLYCOPROTEINS OF TRYPANOSOMA-CRUZI BY TWO-DIMENSIONAL ELECTROPHORESIS - A CORRELATION WITH THE CELL INVASION CAPACITY
    ANDREWS, NW
    KATZIN, AM
    COLLI, W
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (03): : 599 - 604
  • [3] SEROLOGICAL ACTIVITY AGAINST GALACTOSYL-ALPHA-(1-3)GALACTOSE IN SERA FROM PATIENTS WITH SEVERAL KINETOPLASTIDA INFECTIONS
    AVILA, JL
    ROJAS, M
    TOWBIN, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (01) : 126 - 132
  • [4] BHAVANANDAN VP, 1979, J BIOL CHEM, V254, P4000
  • [5] SIALIC-ACID IN A COMPLEX OLIGOSACCHARIDE CHAIN OF THE TC-85 SURFACE GLYCOPROTEIN FROM THE TRYPOMASTIGOTE STAGE OF TRYPANOSOMA-CRUZI
    COUTO, AS
    KATZIN, AM
    COLLI, W
    DELEDERKREMER, RM
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 26 (1-2) : 145 - 153
  • [6] CHARACTERIZATION OF THE MECHANISM OF PROTEIN GLYCOSYLATION AND THE STRUCTURE OF GLYCOCONJUGATES IN TISSUE-CULTURE TRYPOMASTIGOTES AND INTRACELLULAR AMASTIGOTES OF TRYPANOSOMA-CRUZI
    DOYLE, P
    DELACANAL, L
    ENGEL, JC
    PARODI, AJ
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1986, 21 (01) : 93 - 101
  • [7] CARBOHYDRATE ANTIGENS AS POSSIBLE PARASITE VACCINES - A CASE FOR THE LEISHMANIA GLYCOLIPID
    HANDMAN, E
    MCCONVILLE, MJ
    GODING, JW
    [J]. IMMUNOLOGY TODAY, 1987, 8 (06): : 181 - 185
  • [8] NEURAMINIDASE FROM TRYPANOSOMA-CRUZI - ANALYSIS OF ENHANCED EXPRESSION OF THE ENZYME IN INFECTIOUS FORMS
    HARTH, G
    HAIDARIS, CG
    SO, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) : 8320 - 8324
  • [9] HODGES LC, 1979, J BIOL CHEM, V254, P8208
  • [10] MULTIPLE TRYPANOSOMA-CRUZI ANTIGENS CONTAINING TANDEMLY REPEATED AMINO-ACID SEQUENCE MOTIFS
    IBANEZ, CF
    AFFRANCHINO, JL
    MACINA, RA
    REYES, MB
    LEGUIZAMON, S
    CAMARGO, ME
    ASLUND, L
    PETTERSSON, U
    FRASCH, ACC
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 30 (01) : 27 - 34