ARGIOTOXIN DETECTS MOLECULAR DIFFERENCES IN AMPA RECEPTOR CHANNELS

被引：144

作者：

HERLITZE, S

RADITSCH, M

RUPPERSBERG, JP

JAHN, W

MONYER, H

SCHOEPFER, R

WITZEMANN, V

机构：

[1] MAX PLANCK INST MED RES,BIOPHYS ABT,W-6900 HEIDELBERG 1,GERMANY

[2] UNIV HEIDELBERG,CTR MOLEC BIOL,MOLEC NEUROSCI LAB,W-6900 HEIDELBERG,GERMANY

来源：

NEURON | 1993年 / 10卷 / 06期

关键词：

D O I：

10.1016/0896-6273(93)90061-U

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Argiotoxin, a component of the spider venom from Argiope lobata, blocks AMPA receptor channels expressed in homomeric and heteromeric configuration in Xenopus oocytes. Argiotoxin acts as an open channel blocker in a voltage-dependent manner and discriminates between the functionally diverse AMPA receptors. Importantly, a transmembrane region 2 determinant for divalent cation permeability also determines argiotoxin sensitivity. Subunit-specific differences in the time courses of block and recovery demonstrate that heteromeric AMPA receptors can assemble in variable ratios. Thus, argiotoxin can be used as a tool in analyzing the subunit composition of AMPA receptors in native membranes.

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收藏

页码：1131 / 1140

页数：10

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