LIPOSOMES COATED WITH CRYSTALLINE BACTERIAL-CELL SURFACE PROTEIN (S-LAYER) AS IMMOBILIZATION STRUCTURES FOR MACROMOLECULES

被引:88
作者
KUPCU, S
SARA, M
SLEYTR, UB
机构
[1] UNIV WIEN,ZENTRUM ULTRASTRUKTURFORSCH,A-1180 VIENNA,AUSTRIA
[2] UNIV WIEN,LUDWIG BOLTZMANN INST MOLEK NANOTECHNOL,A-1180 VIENNA,AUSTRIA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1995年 / 1235卷 / 02期
关键词
LIPOSOME; SURFACE LAYER; CRYSTALLINE; S LAYER; CELL SURFACE PROTEIN; IMMOBILIZATION; (B-COAGULANS);
D O I
10.1016/0005-2736(95)80013-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Isolated subunits from the crystalline cell surface layer (S-layer) of Bacillus coagulans E38-66 were recrystallized on positively charged liposomes. The liposomes were composed of dipalmitoylphosphatidylcholine/cholesterol and stearylamine. The natural arrangement of the S-layer subunits on the bacterial surface is as an oblique (p2) lattice. The subunits attached to positively charged liposomes by their inner face (which bears a net negative charge) in an orientation identical to the lattice on intact cells. The S-layer protein, once recrystallized on liposomes, was crosslinked with glutaraldehyde and subsequently used as a matrix for the covalent attachment of macromolecules. The high stability of S-layer-coated liposomes and the possibility for immobilizing biologically active molecules on the crystalline array may offer potential in various different liposome applications.
引用
收藏
页码:263 / 269
页数:7
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