GREEN FLUORESCENT PROTEIN-PRODUCTION IN THE CELLULAR SLIME-MOLDS POLYSPHONDYLIUM-PALLIDUM AND DICTYOSTELIUM-DISCOIDEUM

被引：30

作者：

FEY, P ^{[1
]}

COMPTON, K ^{[1
]}

COX, EC ^{[1
]}

机构：

[1] PRINCETON UNIV,DEPT MOLEC BIOL,MOFFETT LABS 333,PRINCETON,NJ 08544

来源：

GENE | 1995年 / 165卷 / 01期

关键词：

GFP; FACS SORTING; MORPHOGENESIS; TRANSFORMATION;

D O I：

10.1016/0378-1119(95)00430-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The green fluorescent protein-encoding gene from Aequorea victoria has been eloped into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FAGS).

引用

页码：127 / 130

页数：4

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