A SIMPLE ASSAY FOR DETERMINATION OF IRON RELEASE FROM FERRITIN IN NEUROBLASTOMA-CELLS

被引：4

作者：

BAADER, SL ^{[1
]}

BRUCHELT, G ^{[1
]}

HANDGRETINGER, R ^{[1
]}

NIETHAMMER, D ^{[1
]}

机构：

[1] UNIV TUBINGEN,CHILDRENS HOSP,DEPT HEMATOL & ONCOL,RUEMELINSTR 23,W-7400 TUBINGEN 1,GERMANY

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 25卷 / 01期

关键词：

CELLULAR FERRITIN; IRON RELEASE; (NEUROBLASTOMA CELL);

D O I：

10.1016/0165-022X(92)90031-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A commercially available enzyme immunoassay was used to determine ferritin content and subsequently the loading and release of iron from ferritin in neuroblastoma cells. LS cells were incubated with Fe-59 for 24 h, lysed, and the cytoplasmic ferritin was bound to monoclonal antibodies coupled to globules. After determination of the ferritin content the same globules with bound radioactive ferritin were measured in a gamma-counter. To illustrate the applicability of this test system, increased iron loading of cellular ferritin could be demonstrated in cycloheximide-treated cells; furthermore, release of iron was documented after incubation of LS cells with a combination of 6-hydroxydopamine and ascorbate. The assay turned out to be a simple method for determination of changes in Fe-59 content of ferritin in neuroblastoma cells.

引用

页码：11 / 15

页数：5

共 10 条

[1] REDUCTIVE RELEASE OF FERRITIN IRON - A KINETIC ASSAY [J].

BOYER, RF ;

GRABILL, TW ;

PETROVICH, RM .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :17-22

[2]

BRUCHELT G, 1991, CANCER RES, V51, P6066

[3]

HALLIWELL B, 1990, METHOD ENZYMOL, V186, P1

[4]

IANCU TC, 1988, CANCER, V61, P2497, DOI 10.1002/1097-0142(19880615)61:12<2497::AID-CNCR2820611218>3.0.CO

[5]

2-P

[6] RELEASE OF IRON FROM FERRITIN BY 6-HYDROXYDOPAMINE UNDER AEROBIC AND ANAEROBIC CONDITIONS [J].

LODE, HN ;

BRUCHELT, G ;

RIETH, AG ;

NIETHAMMER, D .

FREE RADICAL RESEARCH COMMUNICATIONS, 1990, 11 (1-3) :153-158

[7] 6-HYDROXYDOPAMINE RELEASES IRON FROM FERRITIN AND PROMOTES FERRITIN-DEPENDENT LIPID-PEROXIDATION [J].

MONTEIRO, HP ;

WINTERBOURN, CC .

BIOCHEMICAL PHARMACOLOGY, 1989, 38 (23) :4177-4182

[8]

RUDOLPH G, 1991, HUM GENET, V86, P562

[9]

STURROCK A, 1990, J BIOL CHEM, V265, P3139

[10] FERRITIN - STRUCTURE, GENE-REGULATION, AND CELLULAR FUNCTION IN ANIMALS, PLANTS, AND MICROORGANISMS [J].

THEIL, EC .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :289-315

← 1 →