USE OF A DIGOXIGENIN-LABELED RNA PROBE TO DETECT ALL 24 SEROTYPES OF BLUETONGUE VIRUS IN CELL-CULTURE

被引：8

作者：

BROWN, CC

MEYER, RF

GRUBMAN, MJ

机构：

[1] Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service USDA, Greenport, NY 11944

[2] Plum Island Animal Disease Center, Agricultural Research Service USDA, Greenport, NY 11944

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 02期

关键词：

D O I：

10.1177/104063879300500203

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A digoxigenin-labeled RNA probe, corresponding to the section of the bluetongue virus (BTV) serotype 17 genome coding for nonstructural protein-1 (NS1), was applied to noninfected cell cultures and cell cultures infected with 24 different serotypes of BTV, 2 serotypes of epizootic hemorrhagic disease virus, and African horse sickness virus type 4. The probe hybridized to all cell cultures infected with the various BTV serotypes but did not hybridize to noninfected cell cultures or cell cultures infected with any of the other orbiviruses.

引用

页码：159 / 162

页数：4

共 15 条

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