REMOVAL OF LPS FROM A BRUCELLA CYTOPLASMIC FRACTION BY AFFINITY-CHROMATOGRAPHY WITH AN ANTI-LPS MONOCLONAL-ANTIBODY AS IMMUNOSORBENT

被引：14

作者：

GOLDBAUM, FA ^{[1
]}

RUBBI, CP ^{[1
]}

FOSSATI, CA ^{[1
]}

机构：

[1] UBA,CONICET,FAC FARM & BIOQUIM,INST ESTUDIOS INMUNIDAD HUMORAL,RA-1113 BUENOS AIRES,DF,ARGENTINA

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1994年 / 40卷 / 03期

关键词：

D O I：

10.1099/00222615-40-3-174

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Affinity chromatography on polymyxin B-Sepharose 4B is one of the most commonly used methods for the removal of contaminating lipopolysaccharides (LPS). However, the LPS of Brucella spp. do not bind to polymyxin B. An affinity chromatography method with an anti-O antigen of Brucella LPS monoclonal antibody as immunosorbent was developed. The method produced a 1000-fold reduction in the LPS content of the cytoplasmic fraction of B. abortus. The eluted proteins retained their antigenicity. The method, which uses mild physiological conditions, is simple, effective and reproducible.

引用

页码：174 / 178

页数：5

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