ISOLATION AND CHARACTERIZATION OF AN ANTICOAGULANT PRESENT IN THE SALIVARY-GLANDS OF THE BONT-LEGGED TICK, HYALOMMA-TRUNCATUM

被引：37

作者：

JOUBERT, AM

CRAUSE, JC

GASPAR, ARMD

CLARKE, FC

SPICKETT, AM

NEITZ, AWH

机构：

[1] Department of Biochemistry, University of Pretoria, Pretoria

[2] Department of Biology, MEDUNSA

[3] Veterinary Research Institute, Onderstepoort

来源：

EXPERIMENTAL & APPLIED ACAROLOGY | 1995年 / 19卷 / 02期

关键词：

HAEMATOPHAGEOUS; TICK; HYALOMMA TRUNCATUM; ANTICOAGULANT; FACTOR XA;

D O I：

10.1007/BF00052548

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A low molecular mass anticoagulant (17 kDa) was isolated from the salivary glands of prefed female Hyalomma truncatum ticks by means of reverse phase and anion-exchange HPLC. Trypsin digestion and amino acid analysis confirmed the protein nature of the anticoagulant. The inhibitor appears to be uncompetitive with a K-i of 6.9 x 10(-10)M. The target of the anticoagulant is factor Xa at the junction of the extrinsic and intrinsic pathways. This may be crucial for the survival of the tick, making it feasible to investigate the possibility of vaccination with this antihaemostatic against tick feeding. In addition, tick anticoagulants may possibly have therapeutic application in controlling thrombosis.

引用

页码：79 / 92

页数：14

共 14 条

[1]

Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding, Anal. Biochem., 72, pp. 248-254, (1976)

[2]

Cornish-Bowden A., Why is uncompetitive inhibition so rare? A possible explanation with implications for the design of drugs and pesticides, FEBS., 203, pp. 3-6, (1986)

[3]

Gordon J.R., Allen J.R., Factors V and VII anticoagulant activities in the salivary glands of feeding Dermacentor andersoni ticks, J. Parasitol., 77, pp. 167-170, (1991)

[4]

Jordan S.P., Waxman L., Smith D.E., Vlasuk G.P., Tick anticoagulant peptide: kinetic analysis of the recombinant inhibitor with blood coagulation factor Xa, Biochemistry, 29, pp. 11095-11100, (1990)

[5]

Limo M.K., Voigt W.P., Tumbo-Oeri A.G., Njogi R.M., Ole-MoiYoi O.K., Purification and characterization of an anticoagulant from the salivary glands of the ixodid tick, Rhipicephalus appendiculatus, Exp. Parasitol., 72, pp. 418-429, (1991)

[6]

Margolis J., Activation of plasma by contact with glass: evidence for a common reaction which releases plasma kinin and initiates coagulation, J. Physiol., 144, pp. 1-22, (1958)

[7]

Mathews C.K., van Holde K.E., The Regulation of Enzyme Activity, Biochemistry, pp. 397-399, (1990)

[8]

Meyer B.H., Luus H.G., Muller F.O., Badenhorst P.N., Lotter M.G., Rothig H.J., Grotsch H., Rozenkranz B., Recombinant hirudin, a new anticoagulant, has no effect on feacal blood loss, Br. J. Clin. Pharmac., 33, pp. 524-526, (1992)

[9]

Neeper M.P., Waxman L., Smith D.E., Schulman C.A., Sardona M., Ellis R.W., Schaffer L.W., Siegl P.K.S., Vlasuk G.P., Characterization of recombinant tick anticoagulant peptide: a highly selective inhibitor of blood coagulation factor Xa, J. Biol. Chem., 265, pp. 17746-17752, (1990)

[10]

Segal I.H., Enzymes, Biochemical Calculations, (1976)

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