MONTE-CARLO CONVOLUTION METHOD FOR SIMULATION AND ANALYSIS OF FLUORESCENCE DECAY DATA

被引：30

作者：

CHOWDHURY, FN

KOLBER, ZS

BARKLEY, MD

机构：

[1] Department of Chemistry, Louisiana State University, Baton Rouge

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1991年 / 62卷 / 01期

关键词：

D O I：

10.1063/1.1142280

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A Monte Carlo convolution method for simulating time-correlated single photon counting data is presented. The random convolution automatically produces the Poisson statistics of the real experiment. The new simulation technique offers realistic treatment of various aspects of the single photon counting experiment, including pulse pileup. The random convolution is also incorporated in a data analysis technique using a reference fluorophore. Illustrative examples comparing the Monte Carlo and conventional simulation methods are given and the conceptual differences are discussed.

引用

页码：47 / 52

页数：6

共 34 条

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