A HEAT-INDUCIBLE NUCLEAR FACTOR THAT BINDS TO THE HEAT-SHOCK ELEMENT OF THE HUMAN HEME OXYGENASE GENE

被引：23

作者：

MITANI, K ^{[1
]}

FUJITA, H ^{[1
]}

SASSA, S ^{[1
]}

KAPPAS, A ^{[1
]}

机构：

[1] ROCKEFELLER UNIV HOSP,NEW YORK,NY 10021

来源：

BIOCHEMICAL JOURNAL | 1991年 / 277卷

关键词：

D O I：

10.1042/bj2770895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Haem oxygenase is a heat-shock protein in several rat tissues, as well as in certain human cells such as Hep3B hepatoma cells. In common with other heat-shock-protein genes, both the human and the rat haem oxygenase genes contain a heat-shock element (HSE) in their promoter regions. In the present study we have identified a factor in nuclear extracts of human Hep3B cells which binds specifically to the HSE of the human haem oxygenase gene. The factor in Hep3B cells was significantly induced within 1 h after heat-shock treatment, and the induction was blocked by treatment of cells with actinomycin D or cycloheximide. The factor was not detected in human HepG2 hepatoma cells, which exhibit the heat-mediated induction of heat-shock protein 70 mRNA, but not that of haem oxygenase mRNA. These findings suggest that the heat-inducible nuclear factor is increased at the level of transcription and that it may activate the human haem oxygenase gene via the HSE after heat treatment.

引用

页码：895 / 897

页数：3

共 19 条

[1] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].