MODIFICATION OF ALPHA-AMYLASE FROM BACILLUS-LICHENIFORMIS BY THE POLYALDEHYDE DERIVED FROM BETA-CYCLODEXTRINE AND ALPHA-AMYLASE THERMOSTABILITY

被引：10

作者：

MORAND, P ^{[1
]}

BIELLMANN, JF ^{[1
]}

机构：

[1] UNIV STRASBOURG 1,DEPT CHIM,CHIM ORGAN BIOL LAB,CNRS,URA 31,1 RUE BLAISE PASCAL,F-67008 STRASBOURG,FRANCE

来源：

FEBS LETTERS | 1991年 / 289卷 / 02期

关键词：

ALPHA-AMYLASE; BETA-CYCLODEXTRINE; THERMOSTABILITY;

D O I：

10.1016/0014-5793(91)81056-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cleavage of beta-cyclodextrine by sodium periodate at the seven 2-3 diols of the glucose unit gives rise to the polyaldehyde 1, used to modify alpha-amylase. The reductive modification of alpha-amylase from Bacillus licheniformis reduced the number of reactive lysine groups from 8 to 3.5 per mol of enzyme with an activity loss of 25% and increased the half-life at 80-degrees-C from 4.7 to 7.0 minutes.

引用

页码：148 / 150

页数：3

共 13 条

[1] AMYLASES, ALPHA AND BETA [J].