NUCLEOSOMES OCCURRING IN PROTEIN-FREE HYBRIDOMA CELL-CULTURE - EVIDENCE FOR PROGRAMMED CELL-DEATH

被引：69

作者：

FRANEK, F

DOLNIKOVA, J

机构：

[1] Institute of Molecular Genetics, Czechoslovak Academy of Science, CS-14220 Praha 4

来源：

FEBS LETTERS | 1991年 / 284卷 / 02期

关键词：

NUCLEOSOME; CHROMATIN; B-HYDRIDOMA; CELL CULTURE; CELL DEATH; APOPTOSIS;

D O I：

10.1016/0014-5793(91)80705-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In addition to monoclonal immunoglobulin, two kinds of nucleoproteins, NP1 and NP2, were isolated from the supernatants of hybridoma cultures set up in a protein-free medium. As shown by SDS-electrophoresis the two nucleoproteins shared a set of proteins (apparent M(r) 11000 to 15000), and differed in the DNA moiety (approximately 150 bp in NPI, approximately 350 bp in NP2). The amino acid composition of the protein moiety confirmed the nucleosomal origin of NPI and NP2. The findings support the view that in hybridoma cultures the cells undergo death by apoptosis, i.e. a programmed process characterized by initial fragmentation of chromatin.

引用

页码：285 / 287

页数：3

共 13 条

[1] ELECTRON-MICROSCOPY OF HYBRIDOMA CELLS WITH SPECIAL REGARD TO MONOCLONAL-ANTIBODY PRODUCTION [J].