EXPRESSION OF THE INTERNAL CALCIUM-PUMP IN PREGNANT RAT UTERUS

被引：22

作者：

KHAN, I

TABB, T

GARFIELD, RE

JONES, LR

FOMIN, VP

SAMSON, SE

GROVER, AK

机构：

[1] MCMASTER UNIV,DEPT BIOMED SCI,1200 MAIN ST W,HAMILTON L8N 3Z5,ONTARIO,CANADA

[2] MCMASTER UNIV,DEPT MED,HAMILTON L8N 3Z5,ONTARIO,CANADA

[3] UNIV TEXAS,MED BRANCH,DEPT OBSTET & GYNECOL,GALVESTON,TX 77550

[4] INDIANA UNIV,SCH MED,KRANNERT INST CARDIOL,DEPT MED,INDIANAPOLIS,IN 46202

[5] INDIANA UNIV,SCH MED,KRANNERT INST CARDIOL,DEPT BIOCHEM,INDIANAPOLIS,IN 46202

[6] AV PALLADIN BIOCHEM INST,DEPT BIOCHEM KINET,KIEV 252030,UKRAINE

来源：

CELL CALCIUM | 1993年 / 14卷 / 02期

关键词：

D O I：

10.1016/0143-4160(93)90081-G

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Uterine contraction to agents such as oxytocin during labour may utilize Ca2+ sequestered by Ca2+ pump into the sarcoplasmic reticulum (SR). Uterus expresses the SR Ca2+ pump gene SERCA2 as the mRNA splice which encodes the protein SERCA2b. The expression of SERCA2 mRNA and protein was monitored in uteri of day 15 pregnant and delivering rats. The ratio of SERCA2 mRNA to 28S RNA increased by 20% from day 15 to delivery. SERCA2 protein examined by two antibodies increased by 54-55%. Ca2+ dependent acylphosphate intermediate of 115 kD corresponding to SERCA2 also increased 72% during this period. Thus, there is an increase in the SERCA2 expression from day 15 to delivery.

引用

页码：111 / 117

页数：7

共 24 条

[1] INVOLVEMENT OF PROTEIN KINASE-A IN THE REGULATION OF INTRACELLULAR FREE CALCIUM AND PHOSPHOINOSITIDE TURNOVER IN RAT MYOMETRIUM [J].