SELF-ASSEMBLY OF MEDIATOR-MODIFIED ENZYME IN POROUS GOLD-BLACK ELECTRODE FOR BIOSENSING

被引：31

作者：

IMAMURA, M ^{[1
]}

HARUYAMA, T ^{[1
]}

KOBATAKE, E ^{[1
]}

IKARIYAMA, Y ^{[1
]}

AIZAWA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOENGN,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1995年 / 24卷 / 1-3期

关键词：

BIOSENSORS; GOLD-BLACK; MEDIATOR-MODIFIED ENZYME; SELF-ASSEMBLY;

D O I：

10.1016/0925-4005(95)85024-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ferrocene-modified glucose oxidase, in which 6-11 ferrocene molecules are covalently attached to glucose oxidase, is covalently bound to self-assembled aminoethane thiol on a smooth gold electrode surface or a porous gold-black electrode surface. Reversible electron transfer is demonstrated between the ferrocene-modified glucose oxidase and the electrode. Enzymatic oxidation of glucose is efficiently coupled with the electrochemical oxidation of enzyme through the enzyme-bound mediator. The electron transfer of modified enzyme is enhanced by the gold-black electrode, which can provide the modified enzyme with increased electron-transfer sites.

引用

页码：113 / 116

页数：4

共 3 条

[1]

AIZAWA M, 1987, 1ST P INT S EL SYNTH, P353

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DEGANI, Y ;

HELLER, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (08) :2615-2620

[3] ENZYME ENTRAPMENT IN ELECTRICALLY CONDUCTING POLYMERS - IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLYPYRROLE AND ITS APPLICATION IN AMPEROMETRIC GLUCOSE SENSORS [J].

FOULDS, NC ;

LOWE, CR .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1986, 82 :1259-1264

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