HUMAN AND MOUSE MONOCLONAL-ANTIBODIES BY REPERTOIRE CLONING

被引：37

作者：

BURTON, DR ^{[1
]}

机构：

[1] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,KREBS INST,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

TRENDS IN BIOTECHNOLOGY | 1991年 / 9卷 / 05期

关键词：

D O I：

10.1016/0167-7799(91)90055-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antibody repertoires, the wide range of antibody molecules produced by animals, can now be established in bacteria by cloning and expression of antibody genes. Beginning with immunized animals, antigen can be used to select, from the repertoire, clones which secrete specific monoclonal antibody. In the future, immunization may become unnecessary. The method may provide a general route, which has so far eluded biotechnologists, to human monoclonal antibodies.

引用

页码：169 / 175

页数：7

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