THE VIP(2) RECEPTOR - MOLECULAR CHARACTERIZATION OF A CDNA-ENCODING A NOVEL RECEPTOR FOR VASOACTIVE-INTESTINAL-PEPTIDE

被引：489

作者：

LUTZ, EM ^{[1
]}

SHEWARD, WJ ^{[1
]}

WEST, KM ^{[1
]}

MORROW, JA ^{[1
]}

FINK, G ^{[1
]}

HARMAR, AJ ^{[1
]}

机构：

[1] ROYAL EDINBURGH & ASSOCIATED HOSP, EDINBURGH, SCOTLAND

来源：

FEBS LETTERS | 1993年 / 334卷 / 01期

关键词：

VASOACTIVE INTESTINAL PEPTIDE (VIP); G-PROTEIN-LINKED RECEPTOR; RAT PITUITARY; RAT OLFACTORY BULB; CDNA CLONING;

D O I：

10.1016/0014-5793(93)81668-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel VIP receptor which we have designated the VIP2 receptor. In situ hybridisation showed that mRNA for this receptor was present mainly in the thalamus, hippocampus and in the suprachiasmatic nucleus.

引用

页码：3 / 8

页数：6

共 43 条

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