POLYETHENOADENOSINE PHOSPHATE AS A FLUOROGENIC SUBSTRATE FOR BARNASE

被引：10

作者：

FITZGERALD, PC ^{[1
]}

HARTLEY, RW ^{[1
]}

机构：

[1] NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 214卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyethenoadenosine phosphate is rapidly hydrolyzed by barnase with an accompanying large increase in fluorescence. At pH 8, 0.2 M ammonium acetate, the initial rate of fluorescence increase is proportional to barnase concentration and thus provides an excellent quantitative assay for its activity. The preparation and use of this substrate in the barnase assay is presented in detail. © 1993 Academic Press, Inc.

引用

页码：544 / 547

页数：4

共 20 条

[1] KINETIC-STUDIES OF RECA PROTEIN-BINDING TO A FLUORESCENT SINGLE-STRANDED POLYNUCLEOTIDE
CHABBERT, M
CAZENAVE, C
HELENE, C
[J]. BIOCHEMISTRY, 1987, 26 (08) : 2218 - 2225
[2] BARNASE HAS SUBSITES THAT GIVE RISE TO LARGE RATE ENHANCEMENTS
DAY, AG
PARSONAGE, D
EBEL, S
BROWN, T
FERSHT, AR
[J]. BIOCHEMISTRY, 1992, 31 (28) : 6390 - 6395
[3] A FLUORESCENCE STUDY OF THE BINDING OF EUKARYOTIC INITIATION-FACTORS TO MESSENGER-RNA AND MESSENGER-RNA ANALOGS
GOSS, DJ
WOODLEY, CL
WAHBA, AJ
[J]. BIOCHEMISTRY, 1987, 26 (06) : 1551 - 1556
[4] BARNASE AND BARSTAR - 2 SMALL PROTEINS TO FOLD AND FIT TOGETHER
HARTLEY, RW
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (11) : 450 - 454
[5] DIRECTED MUTAGENESIS AND BARNASE-BARSTAR RECOGNITION
HARTLEY, RW
[J]. BIOCHEMISTRY, 1993, 32 (23) : 5978 - 5984
[6] HARTLEY RW, 1972, PREP BIOCHEM, V2, P243, DOI 10.1080/00327487208061474
[7] BARNASE AND BARSTAR - EXPRESSION OF ITS CLONED INHIBITOR PERMITS EXPRESSION OF A CLONED RIBONUCLEASE
HARTLEY, RW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 202 (04) : 913 - 915
[8] HARTLEY RW, 1972, PREP BIOCHEM, V2, P229
[9] THE STRUCTURAL AND SEQUENCE HOMOLOGY OF A FAMILY OF MICROBIAL RIBONUCLEASES
HILL, C
DODSON, G
HEINEMANN, U
SAENGER, W
MITSUI, Y
NAKAMURA, K
BORISOV, S
TISCHENKO, G
POLYAKOV, K
PAVLOVSKY, S
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1983, 8 (10) : 364 - 369
[10] JANIK B, 1973, PHYSIOL CHEM PHYS M, V5, P27

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