INTERNALIZATION OF THE RAT AT(1A) AND AT(1B) RECEPTORS - PHARMACOLOGICAL AND FUNCTIONAL REQUIREMENTS

被引：92

作者：

CONCHON, S ^{[1
]}

MONNOT, C ^{[1
]}

TEUTSCH, B ^{[1
]}

CORVOL, P ^{[1
]}

CLAUSER, E ^{[1
]}

机构：

[1] COLL FRANCE,INSERM,U36,F-75005 PARIS,FRANCE

来源：

FEBS LETTERS | 1994年 / 349卷 / 03期

关键词：

ANGIOTENSIN II; G-PROTEIN COUPLED RECEPTOR; INTERNALIZATION;

D O I：

10.1016/0014-5793(94)00703-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The capacity of the angiotensin II (AngII) agonist [Sar1]AngII, the antagonist [Sar1-Ile8]AngII and the non-peptidic antagonist DuP753 to undergo receptor internalization were studied in Chinese hamster ovary cells expressing rat AngII type 1a or 1b receptors (AT(1a) or AT(1b)) or a mutant of AT(1a) (Asn(74)) unable to couple G-protein. In this expression system, the ligand-induced internalization of rat AT(1a) and AT(1b) are similar. Moreover, peptidic ligands, either the agonist or antagonist, induce a significant internalization of AT(1) receptors, but the non-peptidic antagonist DuP753 is far less potent. Finally, the normal internalization of the mutant Asn(74) demonstrates that receptor activation and G-protein coupling are not required for AT(1a) internalization.

引用

页码：365 / 370

页数：6

共 40 条

[1] MORPHOLOGICAL AND BIOCHEMICAL-ANALYSIS OF ANGIOTENSIN-II INTERNALIZATION IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS [J].