MOLECULAR-CLONING OF A TRANSCRIPTION FACTOR, AGP EBP, THAT BELONGS TO MEMBERS OF THE C/EBP FAMILY

被引:266
作者
CHANG, CJ
CHEN, TT
LEI, HY
CHEN, DS
LEE, SC
机构
[1] ACAD SINICA,INST BIOL CHEM,TAINAN,PEOPLES R CHINA
[2] NATL CHENG KUNG UNIV,SCH MED,DEPT MICROBIOL,TAINAN,TAIWAN
[3] NATL TAIWAN UNIV,SCH MED,GRAD INST CLIN MED,TAIPEI,TAIWAN
关键词
D O I
10.1128/MCB.10.12.6642
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of α1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP and C/EBP share 87% amino acid sequence homology in the 'leucine zipper' and its associated DNA-binding domains, while their sequences outside these domains and the sizes of their mRNAs are different. Unlike the limited expression of C/EBP in tissues and cells, AGP/EBP appears to be ubiquitously expressed in tissues like lung, spleen, kidney, heart, testis, and liver and cell lines like p388D1, 129P (hepatoma cell line of C3H/HeJ), FO (mouse myeloma), and L929. Antibody against cloned and expressed AGP/EBP which was raised in rabbits could recognize AGP/EBP from nuclear extract of a number of cells and tissues. On the basis of our findings about the structural relationship and the similarity of motif recognition, we propose that a family of C/EBP-like transcription factors exists.
引用
收藏
页码:6642 / 6653
页数:12
相关论文
共 43 条