ANALYSIS AND PURIFICATION OF DNA RESTRICTION FRAGMENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:6
作者
MAURI, PL
PIETTA, PG
PACE, M
机构
[1] DIPARTIMENTO SCI & TECNOL BIOMED,SEZ CHIM ORGAN,VIA CELORIA 2,I-20133 MILAN,ITALY
[2] CNR,IST TECNOL BIOMED AVANZATE,I-20131 MILAN,ITALY
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 548卷 / 1-2期
关键词
D O I
10.1016/S0021-9673(01)88609-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The purification and analysis of restriction fragments play a very important role in molecular biology but the traditional assay methods of DNA fragments, based on gel electrophoresis and caesium chloride gradient centrifugation, are time-consuming and difficult to quantify. High-performance liquid chromatography provides an alternative method which allows the direct quantitation of picogram amounts of eluents in short times. In the present work we report the separation of different restriction fragments, the purification of some fragments and the relationship between the length of double-stranded DNA fragments and peak areas.
引用
收藏
页码:281 / 287
页数:7
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