MPO ACTIVITY AND GENERATION OF ACTIVE O2 SPECIES IN LEUKOCYTES FROM POORLY CONTROLLED DIABETIC-PATIENTS

OBJECTIVE - This study was undertaken to determine which part of ROI generation is reduced in the neutrophils from patients with NIDDM. RESEARCH DESIGN AND METHODS - Superoxide anion (O2-) production, LDCL activity in response to opsonized zymosan, and MPO activity were measured in leukocytes of poorly controlled NIDDM patients (FBG > 8.89 mM, HbA1 > 10%). RESULTS - In diabetic subjects, both O2- production and LDCL activity assessed with initial slope gradient and peak value were significantly reduced. MPO activity was also decreased in diabetic subjects, and there was a significant correlation between HbA1 levels and MPO activity of diabetic subjects. CONCLUSIONS - This study demonstrated that every step in leukocyte ROI generation should be reduced in the leukocytes from poorly controlled diabetic patients.