THE USE OF BETA-GALACTOSIDASE AS A TRACER IN IMMUNOCYTOCHEMISTRY

被引：123

作者：

BONDI, A

CHIEREGATTI, G

EUSEBI, V

FULCHERI, E

BUSSOLATI, G

机构：

[1] UNIV BOLOGNA, IST ANAT & ISTOL PATOL, I-40126 BOLOGNA, ITALY

[2] OSPED GEN IMOLA, SERV ANAT PATOL, IMOLA, ITALY

[3] CARLO ERBA SPA, FARMITALIA, RIC DIAGNOST LAB, MILANO, ITALY

[4] UNIV GENOA, IST ANAT & ISTOL PATOL, I-16126 GENOA, ITALY

[5] UNIV TURIN, IST ANAT & ISTOL PATOL, I-10126 TURIN, ITALY

来源：

HISTOCHEMISTRY | 1982年 / 76卷 / 02期

关键词：

D O I：

10.1007/BF00501918

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new immunocytochemical method using .beta.-galactosidase as a tracer is described. The positive staining appears blue on an unstained background. The method which was applied to samples of various human tissues has the high sensitivity and specificity of the immunoperoxidase method and appears to be a practical alternative. The substrate has no carcinogenic activity. Staining is permanent and the sections can be dehydrated and mounted in synthetic media. Enzyme and substrate solutions are stable for several months.

引用

页码：153 / 158

页数：6

共 16 条

[1] HISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON PANCREATIC A-CELLS - EVIDENCE FOR GLUCAGON AND NON-GLUCAGON COMPONENTS OF ALPHA GRANULE [J].