INSITU (6-4)PHOTOPRODUCT DETERMINATION BY LASER CYTOMETRY AND AUTORADIOGRAPHY

被引：22

作者：

MORI, T ^{[1
]}

MATSUNAGA, T ^{[1
]}

CHANG, CC ^{[1
]}

TROSKO, JE ^{[1
]}

NIKAIDO, O ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT PEDIAT & HUMAN DEV,E LANSING,MI 48824

来源：

MUTATION RESEARCH | 1990年 / 236卷 / 01期

关键词：

(6-4)Photoproduct; In situ determination; Laser cytometry; Monoclonal antibody; UV damage;

D O I：

10.1016/0921-8777(90)90037-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The UV-induced (6-4)photoproducts and their repair in individual human cells were quantitatively determined by argon-laser imaging microspectrofluorometry or autoradiography with a well-characterized monoclonal antibody against (6-4)photoproducts. (6-4)Photoproduct induction curves were linear as a function of UV dose, using both methods. The formation of (6-4)photoproducts was detected in the cells irradiated with as low as 10 and 25 J/m2 of UV by autoradiography and laser cytometry, respectively. Normal cells repaired more than 80% of the initial damage within 4 h post irradiation. In contrast, almost no repair was observed in xeroderma pigmentosum cells (complementation group A) within 8 h. © 1990.

引用

页码：99 / 105

页数：7

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