DETERMINATION OF THE ISOELECTRIC POINT OF THE CAPILLARY WALL IN CAPILLARY ELECTROPHORESIS - APPLICATION TO PLASTIC CAPILLARIES

被引：21

作者：

ROHLICEK, V ^{[1
]}

DEYL, Z ^{[1
]}

MIKSIK, I ^{[1
]}

机构：

[1] INST PHYSIOL PRAGUE,PRAGUE 4,CZECH REPUBLIC

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 662卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80523-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A refined method for measuring endoosmotic flow in capillaries based on weighing the amount of liquid transferred is described. The method has been applied to the estimation of isoelectric points of two types of plastic capillaries (polytetrafluoroethylene and polytetrafluoroethylene-polyhexafluoropropylene copolymer). Contrary to expectation both plastic capillaries exhibited considerable electroosmotic flow and changes in hydrodynamic flow resistance without applied voltage. The nature of these phenomena is discussed.

引用

页码：369 / 373

页数：5

共 9 条

[1] HIGH-VOLTAGE CAPILLARY ZONE ELECTROPHORESIS - OPERATING PARAMETERS EFFECTS ON ELECTROENDOSMOTIC FLOWS AND ELECTROPHORETIC MOBILITIES [J].