THE GROE GENE-PRODUCTS OF ESCHERICHIA-COLI ARE DISPENSABLE FOR MUCA(+)B(+)-DEPENDENT UV MUTAGENESIS

被引:7
作者
DONNELLY, CE [1 ]
MURLI, S [1 ]
WALKER, GC [1 ]
机构
[1] MIT,DEPT BIOL 56 621,CAMBRIDGE,MA 02139
关键词
GROEL; GROES; PKM101; MUCAB GENES; MOLECULAR CHAPERONES; UV MUTAGENESIS; PHAGE REACTIVATION;
D O I
10.1016/0027-5107(94)90096-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
UV mutagenesis in Escherichia coli requires the groES(+)EL(+) chaperonins as well as the umuD(+)C(+) SOS-regulated genes. GroES and GroEL appear to be required to stabilize UmuC. The mucA(+)B(+) genes, which are encoded on a broad host range plasmid, are functionally analogous and structurally similar to the umuD(+)C(+) genes of E. coli. While these gene pairs are quite similar, differences have been reported in the functioning of these gene products. We tested whether mucA(+)B(+) function requires the groE(+) gene products as well. We show that mucA(+)B(+)-induced UV mutagenesis, UV resistance, phage reactivation and cold sensitivity do not require the groE(+) heat shock genes. These findings suggest that the requirement of UmuC for groES(+)EL(+) function is not shared by its analog, MucB.
引用
收藏
页码:225 / 233
页数:9
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