A NOVEL CLASS OF CYCLIC BETA-DICARBONYL LEAVING GROUPS AND THEIR USE IN THE DESIGN OF BENZISOTHIAZOLONE HUMAN-LEUKOCYTE ELASTASE INHIBITORS

Human leukocyte elastase (HLE) has been proposed to be a primary mediator of pulmonary emphysema, and inhibitors of this enzyme should be effective in the treatment of emphysema and other pulmonary diseases. We have discovered a novel class of alicyclic and heterocyclic leaving groups which share one common structural feature, a cyclic beta-dicarbonyl. This design concept for leaving groups has not been previously reported. A structure-activity relationship has been developed and the concept extended to several types of alicyclic and heterocyclic beta-dicarbonyl systems. This work led to the identification of a potent (K-1* of 0.066 nM) and tissue stable (in vitro: blood t(1/2) = 160 min, Liver t(1/2) > 240 min) benzisothiazolone HLE inhibitor, WIN 65936 (13b).