THE SYNTHESIS OF INFLUENZA-VIRUS NEGATIVE-STRAND RNA TAKES PLACE IN INSOLUBLE COMPLEXES PRESENT IN THE NUCLEAR MATRIX-FRACTION

被引：38

作者：

LOPEZTURISO, JA

MARTINEZ, C

TANAKA, T

ORTIN, J

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,CANTO BLANCO,E-28049 MADRID,SPAIN

[2] OSAKA UNIV,FAC PHARMACEUT SCI,OSAKA,JAPAN

来源：

VIRUS RESEARCH | 1990年 / 16卷 / 03期

关键词：

Cell fractionation; In vitro replication; Influenza virus; Synthesis; vRNA;

D O I：

10.1016/0168-1702(90)90056-H

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The replication of influenza virus RNA in vitro has been studied by cell fractionation of MDCK-infected cells and characterization of in vitro synthesized RNA. Analysis of the RNA product polarity by liquid hybridization to excess single-stranded DNA probes shows that only the RNP complexes present in the nuclear matrix fraction are able to synthesize negative-polarity RNA. This RNA product has been characterized as authentic vRNA by size analysis, RNase-protection by unlabelled, positive-polarity riboprobes and T1-finterprinting. Priming the in vitro reaction with ApG stimulates preferentially the synthesis of positive-polarity RNA, while ApGpU stimulates both positive and negative-polarity RNA synthesis. © 1990.

引用

页码：325 / 337

页数：13

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