DUAL INHIBITION OF CYCLOOXYGENASE AND LIPOXYGENASE BY 2-ACETYLTHIOPHENE 2-THIAZOLYLHYDRAZONE (CBS-1108) AND EFFECT ON LEUKOCYTE MIGRATION INVIVO

CBS-1108, 2-acetylthiophene 2-thiazolyhydrazone, inhibits 5-lipoxygenase activity in [human and rabbit] polymorphonuclear leukocytes (PMN) (IC50 [median inhibitory concentration] = 2 .times. 10-6 M), 12-lipoxygenase (IC50 = 9 .times. 10-6 M) and cyclooxygenase (IC50 = 2 .times. 10-6 M) in platelets. Inhibitory of the 2 pathways of arachidonic acid cascade could lead to additional beneficial antiinflammatory activity by comparison with classical aspirin-like drugs. Only inhibitors of both cyclooxygenase and lipoxygenase such as NDGA [nordihydroquaiaretic acid] and CBS-1108 inhibit leukocyte migration in an animal model [rat] of acute inflammatory response.