A MICROALBUMINURIA ASSAY USING BROMPHENOL BLUE

被引：22

作者：

JUNG, K

NICKEL, E

PERGANDE, M

机构：

[1] Department of Experimental Organ Transplantation, University Hospital Charité, Humboldt University Berlin

来源：

CLINICA CHIMICA ACTA | 1990年 / 187卷 / 02期

关键词：

Dye binding method with bromphenol blue; Interfering factor; Microalbuminuria; Reference interval;

D O I：

10.1016/0009-8981(90)90343-Q

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A simple microalbuminuria assay using bromphenol blue glycine reagent is described. Urine samples were prepared using gel filtration on Sephadex G-50 minicolumns and absorbance was measured at 610 nm 20 s after mixing 10 parts of eluate and 1 part of reagent. The detection limit of this method was 3 mg/1; within-run and between-run precision was between 0.5 and 4.1% for borderline and raised albumin concentrations. The recovery of albumin added to samples was 98.7 ± 2.5%. Results obtained by this method correlated closely with values obtained by radial immunodiffusion (r = 0.987). The test is cheap (reagent costs about 5 cents) and suitable for the non-specialist laboratory. © 1990.

引用

页码：163 / 172

页数：10

共 26 条

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