SYNTHESIS OF 6-PHOSPHATIDYL-L-ASCORBIC ACID BY PHOSPHOLIPASE-D

被引：43

作者：

NAGAO, A

ISHIDA, N

TERAO, J

机构：

[1] National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki

来源：

LIPIDS | 1991年 / 26卷 / 05期

关键词：

D O I：

10.1007/BF02537205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase D (EC 3.1.4.4) of Streptomyces species was found to catalyze transphosphatidylation to L-ascorbic acid from phosphatidylcholine (PC) in a biphasic reaction system. The product was identified as 1,2-diacyl-sn-glycero-3-phospho-6'-L-ascorbic acid (PA-AsA) by mass spectrometry and nuclear magnetic resonance spectroscopy. The optimal pH of transphosphatidylation was 4.5 and the rate of PA-AsA formation increased as concentrations of L-ascorbic acid increased. The conversion of PC to PA-AsA was greater than 80%. PA-AsA was found to be more resistant to hydrolysis by phospholipase D than was PC.

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页码：390 / 394

页数：5

相关论文

共 32 条

[1] CONVERSION OF RADIOLABELED ETHANOLAMINE PLASMALOGEN INTO THE DIMETHYLETHANOLAMINE AND CHOLINE ANALOG VIA TRANSPHOSPHATIDYLATION BY PHOSPHOLIPASE D FROM CABBAGE [J].

ACHTERBERG, V ;

FRICKE, H ;

GERCKEN, G .

CHEMISTRY AND PHYSICS OF LIPIDS, 1986, 41 (3-4) :349-353

[2] THE ANTIOXIDANT ROLE OF VITAMIN-C [J].

BENDICH, A ;

MACHLIN, LJ ;

SCANDURRA, O ;

BURTON, GW ;

WAYNER, DDM .

ADVANCES IN FREE RADICAL BIOLOGY AND MEDICINE, 1986, 2 (02) :419-444

[3] HALO LIPIDS .10. SYNTHESIS AND CYTOSTATIC ACTIVITY OF O-ALKYLGLYCEROPHOSPHO-L-SERINE ANALOGS [J].

BRACHWITZ, H ;

LANGEN, P ;

DUBE, G ;

SCHILDT, J ;

PALTAUF, F ;

HERMETTER, A .

CHEMISTRY AND PHYSICS OF LIPIDS, 1990, 54 (02) :89-98

[4] ENZYMATIC-SYNTHESIS OF PHOSPHATIDYLSERINE AND PURIFICATION BY CM-CELLULOSE COLUMN CHROMATOGRAPHY [J].

COMFURIUS, P ;

ZWAAL, RFA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 488 (01) :36-42

[5] FORMATION OF PHOSPHATIDYLGLYCEROL AND OTHER PHOSPHOLIPIDS BY TRANSFERASE ACTIVITY OF PHOSPHOLIPASE D [J].

DAWSON, RMC .

BIOCHEMICAL JOURNAL, 1967, 102 (01) :205-&

[6]

Eibl H, 1981, Methods Enzymol, V72, P632

[7] SYNTHETIC 1-ACYLGLYCEROPHOSPHOLIPID ANALOGS AS LIPID SUBSTRATES .1. STUDIES ON MICROSOMAL ACYLATION AND UPTAKE INTO LIVER SLICES OF 1-ACYL ANALOGS CONTAINING VARIOUS POLAR BASES [J].

JEZYK, PF ;

HUGHES, HN .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 296 (01) :24-33

[8] KINETIC EVALUATION OF CONVERSION OF PHOSPHATIDYLCHOLINE TO PHOSPHATIDYLETHANOLAMINE BY PHOSPHOLIPASE-D FROM DIFFERENT SOURCES [J].

JUNEJA, LR ;

KAZUOKA, T ;

YAMANE, T ;

SHIMIZU, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 960 (03) :334-341

[9] STUDIES ON ENZYMATIC CONVERSION OF PHOSPHOLIPIDS .6. CONVERSION OF PHOSPHATIDYLCHOLINE TO PHOSPHATIDYLSERINE BY VARIOUS PHOSPHOLIPASES-D IN THE PRESENCE OF L-SERINE OR D-SERINE [J].

JUNEJA, LR ;

KAZUOKA, T ;

GOTO, N ;

YAMANE, T ;

SHIMIZU, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1003 (03) :277-283

[10]

JUNEJA LR, 1987, APPL MICROBIOL BIOT, V27, P146

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