A NOVEL COMBINATION OF PROSTHETIC GROUPS IN A FUNGAL LACCASE - PQQ AND 2 COPPER ATOMS

被引：57

作者：

KARHUNEN, E

NIKUPAAVOLA, ML

VIIKARI, L

HALTIA, T

VANDERMEER, RA

DUINE, JA

机构：

[1] VTT, BIOTECH LAB, TIETOTIE 2, SF-02150 ESPOO, FINLAND

[2] UNIV HELSINKI, DEPT MED CHEM, SF-00100 HELSINKI 10, FINLAND

[3] DELFT UNIV TECHNOL, DEPT MICROBIOL & ENZYMOL, DELFT, NETHERLANDS

来源：

FEBS LETTERS | 1990年 / 267卷 / 01期

关键词：

Copper enzyme; Laccase; Lignin degradation; PQQ; White rot fungus;

D O I：

10.1016/0014-5793(90)80273-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols. © 1990.

引用

页码：6 / 8

页数：3

共 15 条

[1] LOW-TEMPERATURE MAGNETIC CIRCULAR-DICHROISM STUDIES OF NATIVE LACCASE - SPECTROSCOPIC EVIDENCE FOR EXOGENOUS LIGAND BRIDGING AT A TRINUCLEAR COPPER ACTIVE-SITE [J].