TRYPANOSOMA-BRUCEI EVANSI - DYSKINETOPLASIA AND LOSS OF INFECTIVITY AFTER LONG-TERM INVITRO CULTIVATION

被引：21

作者：

ZWEYGARTH, E

KAMINSKY, R

WEBSTER, P

机构：

[1] KENYA TRYPANOSOMIASIS RES INST, KIKUYU, KENYA

[2] FREE UNIV BERLIN, INST PARASITOL & TROP VET MED, W-1000 BERLIN 33, GERMANY

[3] INT LAB RES ANIM DIS, NAIROBI, KENYA

来源：

ACTA TROPICA | 1990年 / 48卷 / 02期

关键词：

Cultivation in vitro; Drug susceptibility; Dyskinetoplasia; Infectivity; Trypanosoma brucei evansi;

D O I：

10.1016/0001-706X(90)90048-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Bloodstream forms of a stock of Trypanosoma brucei evansi were propagated in vitro for more than 14 months. After that period, all organisms were dyskinetoplastic and had lost their infectivity for mice. An increase in diminazene aceturate resistance in vitro was observed whereas the susceptibility to isometamidium chloride, quinapyramine sulphate and suramin was unaltered. Trypanosomes derived from the long term culture had a surface coat. © 1990.

引用

收藏

页码：95 / 99

页数：5

相关论文

共 18 条

[1] CULTIVATION IN A SEMI-DEFINED MEDIUM OF ANIMAL INFECTIVE FORMS OF TRYPANOSOMA-BRUCEI, TRYPANOSOMA-EQUIPERDUM, TRYPANOSOMA-EVANSI, TRYPANOSOMA-RHODESIENSE AND T-GAMBIENSE [J].

BALTZ, T ;

BALTZ, D ;

GIROUD, C ;

CROCKETT, J .

EMBO JOURNAL, 1985, 4 (05) :1273-1277

[2] INVITRO CULTIVATION OF BLOOD-STREAM FORMS OF TRYPANOSOMA-BRUCEI, TRYPANOSOMA-RHODESIENSE, AND TRYPANOSOMA-GAMBIENSE [J].

BRUN, R ;

JENNI, L ;

SCHONENBERGER, M ;

SCHELL, KF .

JOURNAL OF PROTOZOOLOGY, 1981, 28 (04) :470-479

[3] CYSTEINE ELIMINATES THE FEEDER CELL REQUIREMENT FOR CULTIVATION OF TRYPANOSOMA-BRUCEI BLOOD-STREAM FORMS INVITRO [J].

DUSZENKO, M ;

FERGUSON, MAJ ;

LAMONT, GS ;

RIFKIN, MR ;

CROSS, GAM .

JOURNAL OF EXPERIMENTAL MEDICINE, 1985, 162 (04) :1256-1263

[4] GROWTH OF INFECTIVE FORMS OF TRYPANOSOMA-RHODESIENSE INVITRO, CAUSATIVE AGENT OF AFRICAN TRYPANOSOMIASIS [J].

HILL, GC ;

SHIMER, SP ;

CAUGHEY, B ;

SAUER, LS .

SCIENCE, 1978, 202 (4369) :763-765

[5] AFRICAN TRYPANOSOMES - CULTIVATION OF ANIMAL-INFECTIVE TRYPANOSOMA-BRUCEI INVITRO [J].

HIRUMI, H ;

DOYLE, JJ ;

HIRUMI, K .

SCIENCE, 1977, 196 (4293) :992-994

[6] THE LOSS OF THE KINETOPLAST IN TRYPANOSOMES, WITH SPECIAL REFERENCE TO TRYPANOSOMA-EVANSI [J].

HOARE, CA .

JOURNAL OF PROTOZOOLOGY, 1954, 1 (01) :28-33

[7] FEEDER LAYER-FREE INVITRO ASSAY FOR SCREENING ANTITRYPANOSOMAL COMPOUNDS AGAINST TRYPANOSOMA-BRUCEI-BRUCEI AND TRYPANOSOMA-BRUCEI-EVANSI [J].

KAMINSKY, R ;

ZWEYGARTH, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (06) :881-885

[8]

KAMINSKY R, 1988, STABILITY IN VITRO C, P39

[9] IMPROVED FIELD DIAGNOSTIC-TECHNIQUE FOR TRYPANOSOMIASIS BY USE OF A MINICENTRIFUGE [J].

KELLEY, S ;

SCHILLINGER, D .

VETERINARY RECORD, 1983, 113 (10) :219-219

[10] SHORT TERM CULTIVATION OF TRYPANOSOMA BRUCEI IN VITRO AT 37 DEGREES C [J].

LEPAGE, RWF .

NATURE, 1967, 216 (5120) :1141-+