NEW MOLD STARTER CULTURES BY GENETIC-MODIFICATION

被引：10

作者：

GEISEN, R

STANDNER, L

LEISTNER, L

机构：

[1] Bundesanstalt für Fleischforschung, Institut für Mikrobiolagie, 8650, Kulmbach, Toxikologie und Histalogie E. C. Baumannstr

来源：

FOOD BIOTECHNOLOGY | 1990年 / 4卷 / 01期

关键词：

D O I：

10.1080/08905439009549762

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillium nalgiovense is a filamentous fungus which is used in the production of mould fermented meat products. An expression vector with fungal transcription initiation and termination sites was constructed in order to analyse heterologous gene expression in this host. Under the control of these transcription signals the expression of the Escherichia coli β-galactosidase gene could be shown. Penicillium nalgiovense was cotransformed with p3SR2 and pLys 1 which carries the lysostaphin gene from Staphylococcus staphylolyticus. Of the resulting transformants one transformant could be obtained which suppresses the growth of Staphylococcus aureus cells. A DNA fragment of Penicillium nalglovense could be isolated which confers extracellular proteolytic activity to Escherichia coli cells. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：497 / 503

页数：7

共 24 条

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