SEMIQUANTITATIVE DETERMINATION OF H-TYPE-1 AND TYPE-2 ANTIGENS ON BUCCAL EPITHELIAL-CELLS AND IN SALIVA OF SECRETORS AND NONSECRETORS

被引：14

作者：

RAHAT, A ^{[1
]}

STEWART, J ^{[1
]}

BLACKWELL, CC ^{[1
]}

WEIR, DM ^{[1
]}

机构：

[1] UNIV EDINBURGH,SCH MED,DEPT BACTERIOL,EDINBURGH EH8 9AG,MIDLOTHIAN,SCOTLAND

来源：

VOX SANGUINIS | 1990年 / 59卷 / 02期

关键词：

Buccal epithelial cells; H Type 1; H Type 2; Secretor status;

D O I：

10.1111/j.1423-0410.1990.tb05019.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Abstract. The amount of H antigens on buccal epithelial cells (BEC) of secretors and non‐secretors was measured by flow cytometry. H type 1 and H type 2 on BEC were detected with fluorescein‐labelled Ulex europaeus lectin, and H type 2 was measured with mouse monoclonal antibody. Between 3.5 and 6 times more H antigen was detected on cells from secretors compared with cells from non‐secretors. The level of H type 2 was the same on BEC of secretors and non‐secretors. Adsorption of H type 1 antigen from secretor saliva onto non‐secretors BEC was demonstrated. A simple and sensitive ELISA method was developed for the determination of H in saliva with biotin‐labelled Ulex lectin. This system provides a simple means for measuring the amount of H present in saliva and for the determination of secretor status. © 1990 S. Karger AG, Basel

引用

页码：101 / 105

页数：5

共 19 条

[1]

BLACKWELL C C, 1989, FEMS (Federation of European Microbiological Societies) Microbiology Immunology, V47, P341, DOI 10.1016/0378-1097(89)90256-5

[2] ABH AND RELATED HISTO-BLOOD GROUP ANTIGENS - IMMUNOCHEMICAL DIFFERENCES IN CARRIER ISOTYPES AND THEIR DISTRIBUTION [J].

CLAUSEN, H ;

HAKOMORI, S .

VOX SANGUINIS, 1989, 56 (01) :1-20

[3] CARBOHYDRATE CHAINS SPECIFIC FOR BLOOD-GROUP ANTIGENS IN DIFFERENTIATION OF HUMAN ORAL EPITHELIUM [J].

DABELSTEEN, E ;

VEDTOFTE, P ;

HAKOMORI, S ;

YOUNG, WW .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1982, 79 (01) :3-7

[4]

DABELSTEEN E, 1973, ACTA PATH MICRO IM A, VA 81, P435

[5]

DABELSTEEN E, 1971, Scandinavian Journal of Dental Research, V79, P387

[6]

DAVIDSOHN I, 1971, CANCER RES, V31, P1244

[7]

DAVIDSOHN I, 1969, ARCH PATHOL, V87, P306

[8]

DAVIDSOHN I, 1966, ARCH PATHOL, V81, P381

[9] DIFFERENCES IN H ANTIGEN ON HUMAN BUCCAL CELLS FROM SECRETOR AND NON-SECRETOR INDIVIDUALS [J].

FLORY, LL .

VOX SANGUINIS, 1966, 11 (02) :137-&

[10] MOLECULAR RECOGNITION .3. THE BINDING OF THE H-TYPE-2 HUMAN-BLOOD GROUP DETERMINANT BY THE LECTIN-I OF ULEX-EUROPAEUS [J].

HINDSGAUL, O ;

KHARE, DP ;

BACH, M ;

LEMIEUX, RU .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1985, 63 (10) :2653-2658

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