MASS-SPECTROMETRIC INVESTIGATION OF THE PRESENCE OF 7-METHYL RING-OPENED GUANINE DERIVATIVES IN URINE

被引：7

作者：

BARAK, R ^{[1
]}

VINCZE, A ^{[1
]}

BEL, P ^{[1
]}

DUTTA, SP ^{[1
]}

CHEDDA, GB ^{[1
]}

机构：

[1] ROSWELL PK CANC INST,DEPT BIOPHYS,BUFFALO,NY 14263

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1993年 / 86卷 / 01期

关键词：

RING-OPENED; 7-METHYLGUANINE; NUCLEIC ACID ADDUCTS; MASS SPECTROMETRY;

D O I：

10.1016/0009-2797(93)90109-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The involvement of chemical alkylating agents in tumorigenesis and chemotherapy is well established and it was shown that one of the main sites of alkylation is the N-7 of guanine in DNA. Though excission of damaged bases is regarded as one of the repair mechanisms in damaged DNA there is a scarcity of information concerning the excised final metabolites in body fluids. This study attempts to demonstrate the usefulness of CAD MS/MS for the detection of the final metabolite-deformylated ring-opened 7 alkylguanine in urine. Such mass spectrometric methods can be used in biomedical studies.

引用

页码：29 / 40

页数：12

共 37 条

[1]

ANDRIEVSKY GV, 1991, BIOL MASS SPECTROM, V20, P68

[2]

BARAK R, 1990, 38TH P ASMS C TUCS, P814

[3]

BARANEK DT, 1983, BIOCHEM BIOPH RES CO, V110, P625

[4]

CHETSANGA CJ, 1982, CANCER RES, V42, P2616