MINIATURIZED THIN-FILM GLUTAMATE AND GLUTAMINE BIOSENSORS

被引：49

作者：

MOSER, I ^{[1
]}

JOBST, G ^{[1
]}

ASCHAUER, E ^{[1
]}

SVASEK, P ^{[1
]}

VARAHRAM, M ^{[1
]}

URBAN, G ^{[1
]}

ZANIN, VA ^{[1
]}

TJOUTRINA, GY ^{[1
]}

ZHARIKOVA, AV ^{[1
]}

BEREZOV, TT ^{[1
]}

机构：

[1] RUSSIAN PEOPLES FRIENDSHIP UNIV,MOSCOW,RUSSIA

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 6-7期

关键词：

BIOSENSORS; GLUTAMINE; GLUTAMATE; GLUTAMINASE; GLUTAMATE OXIDASE; MINIATURIZED SENSORS; THIN FILM TECHNOLOGY; INTEGRATED SENSORS; MICRO FLOW CELL;

D O I：

10.1016/0956-5663(95)96928-R

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Integrated thin film biosensors were developed for the simultaneous measurement of L-glutamine and L-glutamate in a mu-flow cell. Due to a novel glutaminase with an activity optimum in the neutral pH range, direct monitoring of glutamine in a mammalian cell culture medium could be performed. The glutamine bienzyme sensor was prepared by co-immobilization of glutaminase with glutamate oxidase within a photopatterned poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel membrane. The sensor response was linear in the concentration range of 50 mu mol to 10 mmol glutamine/l. Additionally, a glutamate biosensor was integrated on the sensor chip for difference measurement of possible glutamate interferences. The sensor-chip could be used for at least 300 measurements without any alteration in the performance of its sensors. A new sensor-chip with an integrated how cell provided the possibility of simultaneous measurement of four different parameters at a cell volume of 1 mu l. In order to complete the microsystem, and in order to obtain a ''lab on chip'', a battery operated surface mounted device (SMD) potentiostat was developed.

引用

页码：527 / 532

页数：6

共 13 条

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