DELAYED EXPOSURE TO PULSATILE SHEAR-STRESS IMPROVES RETENTION OF HUMAN SAPHENOUS-VEIN ENDOTHELIAL-CELLS ON SEEDED EPTFE GRAFTS

被引:56
作者
MIYATA, T [1 ]
CONTE, MS [1 ]
TRUDELL, LA [1 ]
MASON, D [1 ]
WHITTEMORE, AD [1 ]
BIRINYI, LK [1 ]
机构
[1] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT SURG,75 FRANCIS ST,BOSTON,MA 02115
关键词
D O I
10.1016/0022-4804(91)90029-L
中图分类号
R61 [外科手术学];
学科分类号
摘要
Since significant loss of endothelial cells (ECs) from the surface of a seeded prosthetic graft occurs after implantation, improved cell retention following exposure to flow should increase the likelihood of long-term success with this technology. An in vitro pulsatile flow circuit was developed to study the effects of two variables on cell retention: cell density at the time of seeding and postseeding incubation time. Fibronectin-coated ePTFE grafts (4 mm × 5 cm) were seeded with human saphenous vein ECs at two densities, confluent (1 × 105 cells/cm2) or subconfluent (2 × 104), and incubated in vitro for varying time intervals (90 min, 1, 3, or 7 days). Test grafts were exposed to 90 min of pulsatile flow in an in vitro flow circuit, then fixed, and stained, and in situ cell counts (cells/cm2) were determined for nine representative fields per graft. Paired control grafts were treated identically but were not exposed to flow. Cell retention was calculated using the formula: % retention = cells/cm2 perfused graft divided by cells/cm2 control graft. Grafts exposed to flow 90 min after seeding demonstrated significantly lower cell retention when compared to later time points. When cells were seeded at confluent density, maximal retention (92 ± 3%) occurred 24 hr after seeding. Prolonged culture of cells seeded on ePTFE grafts at confluent density resulted in increased cell loss. In contrast, on grafts seeded at subconfluent density, retention improved as cells grew to confluence (16 ± 4.5% initially to 82 ± 7% at 7 days). Thus, cell retention on grafts exposed to flow conditions shortly after seeding, whether at confluent or subconfluent density, is significantly worse than identical grafts in which cells are allowed to attach for longer periods. Maximal retention is achieved at confluence, regardless of initial seeding density. © 1991.
引用
收藏
页码:485 / 493
页数:9
相关论文
共 33 条
[1]  
AELBERTYS B, 1989, MOL BIOL CELL, P824
[2]   INFLUENCE OF ENDOTHELIAL-CELL SEEDING ON PLATELET DEPOSITION AND PATENCY IN SMALL-DIAMETER DACRON ARTERIAL GRAFTS [J].
ALLEN, BT ;
LONG, JA ;
CLARK, RE ;
SICARD, GA ;
HOPKINS, KT ;
WELCH, MJ .
JOURNAL OF VASCULAR SURGERY, 1984, 1 (01) :224-233
[3]   OBSERVATIONS ON HUMAN SMOOTH-MUSCLE CELL-CULTURES FROM HYPERPLASTIC LESIONS OF PROSTHETIC BYPASS GRAFTS - PRODUCTION OF A PLATELET-DERIVED GROWTH-FACTOR LIKE MITOGEN AND EXPRESSION OF A GENE FOR A PLATELET-DERIVED GROWTH-FACTOR RECEPTOR - A PRELIMINARY-STUDY [J].
BIRINYI, LK ;
WARNER, SJC ;
SALOMON, RN ;
CALLOW, AD ;
LIBBY, P .
JOURNAL OF VASCULAR SURGERY, 1989, 10 (02) :157-165
[4]   IMPACT OF ENDOTHELIAL-CELL SEEDING ON LONG-TERM PATENCY AND SUBENDOTHELIAL PROLIFERATION IN A SMALL-CALIBER HIGHLY POROUS POLYTETRAFLUOROETHYLENE GRAFT [J].
DOUVILLE, EC ;
KEMPCZINSKI, RF ;
BIRINYI, LK ;
RAMALANJAONA, GR .
JOURNAL OF VASCULAR SURGERY, 1987, 5 (04) :544-550
[5]   RESPONSE OF CULTURED ENDOTHELIAL-CELLS TO STEADY FLOW [J].
ESKIN, SG ;
IVES, CL ;
MCINTIRE, LV ;
NAVARRO, LT .
MICROVASCULAR RESEARCH, 1984, 28 (01) :87-94
[6]   HUMAN-ENDOTHELIAL CELL SEEDING - EVALUATION OF ITS EFFECTIVENESS BY PLATELET PARAMETERS AFTER ONE YEAR [J].
FASOL, R ;
ZILLA, P ;
DEUTSCH, M ;
GRIMM, M ;
FISCHLEIN, T ;
LAUFER, G .
JOURNAL OF VASCULAR SURGERY, 1989, 9 (03) :432-436
[7]   ADULT HUMAN-ENDOTHELIAL CELL COVERAGE OF SMALL-CALIBER DACRON AND POLYTETRAFLUOROETHYLENE VASCULAR PROSTHESES INVITRO [J].
FOXALL, TL ;
AUGER, KR ;
CALLOW, AD ;
LIBBY, P .
JOURNAL OF SURGICAL RESEARCH, 1986, 41 (02) :158-172
[8]  
GRAHAM LM, 1985, ASAIO J, V8, P65
[9]  
HASSON JE, 1986, SURGERY, V100, P884
[10]   ENDOTHELIAL SEEDING OF POLYTETRAFLUOROETHYLENE POPLITEAL BYPASSES - A PRELIMINARY-REPORT [J].
HERRING, MB ;
COMPTON, RS ;
LEGRAND, DR ;
GARDNER, AL ;
MADISON, DL ;
GLOVER, JL .
JOURNAL OF VASCULAR SURGERY, 1987, 6 (02) :114-118